PRODUCT DETAILS Host: Mouse Isotype: IgG1, kappa Clonality: Monoclonal Clone: 3.9 Format: Alexa Fluor 700 Reactivity: Hu Application: Flow Cytometry Tested Dilution: 5 µL (1 µg)/test Concentration: 5 μL/Test Storage: 4°C, store in dark, DO NOT FREEZE! Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2 Purification: Affinity chromatography Data Sheet: TDS Specific Information Description: The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alphaX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. Applications Reported: This 3.9 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
