PRODUCT DETAILS Host: Mouse Isotype: IgG1, kappa Clonality: Monoclonal Clone: 10.1 Format: FITC Reactivity: Hu Application: Flow Cytometry Tested Dilution: 5 µL (1 µg)/test Concentration: 5 μL/Test Storage: 4°C, store in dark, DO NOT FREEZE! Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2 Purification: Affinity chromatography Data Sheet: TDS Specific Information Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative. Applications Reported: The 10.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 10.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
