PRODUCT DETAILS Host: Mouse Isotype: IgG1, kappa Clonality: Monoclonal Clone: UCHT1 Format: NovaFluor Yellow 610 Reactivity: Hu Application: Flow Cytometry Tested Dilution: 5 µL (0.4 µg)/test Concentration: 4 μL/Test Storage: 4°C, store in dark, DO NOT FREEZE! Formulation: PBS with 0.09% sodium azide; pH 7.2 Purification: Affinity chromatography Data Sheet: TDS Specific Information Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer . Applications Reported: The UCHT1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at (5 µL (0.4 µg)/test). A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells. NovaFluor conjugates are based on Phiton technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow) Laser For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
